FIGURE 5.
Inhibition of BCL6 reverses suppression of pro-inflammatory chemokine/cytokine expression in KLF6-deficient macrophages. A–F, BMDMs derived from Lyz2cre, Lyz2cre:KLF6fl/fl mice were nucleofected with BCL6-specific siRNA or control siRNA. These cells were stimulated with 100 ng/ml LPS for 4 h. Total RNA from these experiments was collected, and cDNA was analyzed for expression of Ccl2 (A), Ccl7 (B), Il1α (C), Il1β (D), Il6 (E), and Il12a (F) by quantitative PCR and normalized to 36B4. G, BCL6 protein expression levels in BMDMs following siBcl6 siRNA transfection were analyzed by Western blotting. Western blots were subjected to densitometry analysis utilizing Quantity One 1-D analysis software and normalized to β-Actin. Each experiment was performed four times with six replicates. Graphs represent mean ± S.D. Box plots represent the median with first and third quartiles, and whiskers represent minimum/maximum. *, p ≤ 0.05; N.S., not significant. p < 0.05 between indicated groups is considered significant.