FIGURE 6.
Pharmacological inhibition of BCL6 reverses suppression of pro-inflammatory chemokine/cytokine expression in KLF6-deficient macrophages. A–E, BMDMs derived from Lyz2cre and Lyz2cre:KLF6fl/fl mice were treated with the BCL6-specific inhibitor 79-6 (250 μm). These cells were stimulated with 100 ng/ml LPS for 4 h. Total RNA from these experiments was collected, and cDNA was analyzed for expression of Ccl2 (A), Il1α (B), Il1β (C), Il6 (D), and Il12a (E) by quantitative PCR and normalized to 36B4. Each experiment was performed four times with six replicates. Graphs represent mean ± S.D. *, p ≤ 0.05; N.S., not significant. p < 0.05 between indicated groups is considered significant.