TABLE 1.
Amino acid analysis of human four-protein complex
The experimental amino acid composition of purified complex, as determined via amino acid analysis, is compared with three expected compositions based on the three potential FXN42–210/ISCU/NFS1/ISD11 molar ratios shown. For each molar ratio, the variation between the experimental and expected number of each amino acid and the total % variation is shown. Sixteen amino acids were quantified; tryptophan and cysteine are degraded by the hydrolysis process, and asparagine and glutamine are detected as aspartic and glutamic acid, respectively (75).
Amino Acid | Experimental composition (no. of amino acids) | FXN-ISCU-NFS1-ISD11 (1:1:1:1) |
FXN-ISCU-NFS1-ISD11 (1:1:2:1) |
FXN-ISCU-NFS1-ISD11 (1:1:1:2) |
|||
---|---|---|---|---|---|---|---|
Expected composition (no. of amino acids) | Variation | Expected composition (no. of amino acids) | Variation | Expected composition (no. of amino acids) | Variation | ||
Ala | 70.8 | 63 | −7.8 | 93 | 22.2 | 69 | −1.8 |
Arg | 51.0 | 49 | −2.0 | 76 | 25.0 | 62 | 11.0 |
Asp | 78.9 | 72 | −6.9 | 102 | 23.1 | 82 | 3.1 |
Glu | 96.8 | 85 | −11.8 | 131 | 34.2 | 93 | −3.8 |
Gly | 62.3 | 60 | −2.3 | 92 | 29.7 | 62 | −0.3 |
His | 25.8 | 28 | 2.2 | 47 | 21.2 | 29 | 3.2 |
Ile | 44.6 | 46 | 1.4 | 76 | 31.4 | 52 | 7.4 |
Leu | 75.7 | 76 | 0.3 | 111 | 35.3 | 83 | 7.3 |
Lys | 55.1 | 61 | 5.9 | 82 | 26.9 | 67 | 11.9 |
Met | 21.6 | 24 | 2.4 | 39 | 17.4 | 27 | 5.4 |
Phe | 18.3 | 17 | −1.3 | 24 | 5.7 | 19 | 0.7 |
Pro | 30.4 | 35 | 4.6 | 55 | 24.6 | 37 | 6.6 |
Ser | 60.9 | 67 | 6.1 | 104 | 43.1 | 71 | 10.1 |
Thr | 46.6 | 45 | −1.6 | 65 | 18.4 | 49 | 2.4 |
Tyr | 29.7 | 30 | 0.3 | 45 | 15.3 | 35 | 5.3 |
Val | 58.5 | 59 | 0.5 | 91 | 32.5 | 66 | 7.5 |
Total amino acids | 827.2 | 817 | −10.2 | 1233 | 405.8 | 903 | 75.8 |
Total percent variation | 1.20% | 32.90% | 8.40% |