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. 2016 Aug 12;291(40):21296–21321. doi: 10.1074/jbc.M116.738542

TABLE 1.

Amino acid analysis of human four-protein complex

The experimental amino acid composition of purified complex, as determined via amino acid analysis, is compared with three expected compositions based on the three potential FXN42–210/ISCU/NFS1/ISD11 molar ratios shown. For each molar ratio, the variation between the experimental and expected number of each amino acid and the total % variation is shown. Sixteen amino acids were quantified; tryptophan and cysteine are degraded by the hydrolysis process, and asparagine and glutamine are detected as aspartic and glutamic acid, respectively (75).

Amino Acid Experimental composition (no. of amino acids) FXN-ISCU-NFS1-ISD11 (1:1:1:1)
FXN-ISCU-NFS1-ISD11 (1:1:2:1)
FXN-ISCU-NFS1-ISD11 (1:1:1:2)
Expected composition (no. of amino acids) Variation Expected composition (no. of amino acids) Variation Expected composition (no. of amino acids) Variation
Ala 70.8 63 −7.8 93 22.2 69 −1.8
Arg 51.0 49 −2.0 76 25.0 62 11.0
Asp 78.9 72 −6.9 102 23.1 82 3.1
Glu 96.8 85 −11.8 131 34.2 93 −3.8
Gly 62.3 60 −2.3 92 29.7 62 −0.3
His 25.8 28 2.2 47 21.2 29 3.2
Ile 44.6 46 1.4 76 31.4 52 7.4
Leu 75.7 76 0.3 111 35.3 83 7.3
Lys 55.1 61 5.9 82 26.9 67 11.9
Met 21.6 24 2.4 39 17.4 27 5.4
Phe 18.3 17 −1.3 24 5.7 19 0.7
Pro 30.4 35 4.6 55 24.6 37 6.6
Ser 60.9 67 6.1 104 43.1 71 10.1
Thr 46.6 45 −1.6 65 18.4 49 2.4
Tyr 29.7 30 0.3 45 15.3 35 5.3
Val 58.5 59 0.5 91 32.5 66 7.5
Total amino acids 827.2 817 −10.2 1233 405.8 903 75.8
Total percent variation 1.20% 32.90% 8.40%
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