KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Flag | Sigma-Aldrich | Cat#F3165 |
| Rabbit polyclonal anti-HA | Santa Cruz | Cat#SC-805 |
| Goat polyconal anti-TIA1 | Santa Cruz | Cat#SC-1751 |
| Mouse polyclonal anti-coilin | Abcam | Cat#AB-167253 |
| Rabbit polyclonal anti-SC35 | Santa Cruz | Cat# SC-28720 |
| Rabbit monoclonal anti-vimentin | Cell-signaling | Cat#5741S |
| Mouse monoclonal anti-keratin | Cell-signaling | Cat#4545S |
| Mouse monoclonal anti-tubulin | Sigma-Aldrich | Cat#T5168 |
| Goat-anti-rabbit IgG-HRP conjugate | Bio-rad | Cat#170-6515 |
| Goat-anti-mouse IgG-HRP conjugate | Bio-rad | Cat#172-1011 |
| AlexaFluor 488 conjugated donkey anti-goat IgG | Thermo Fisher Scientific | Ref# A11055 |
| AlexaFluor 488 conjugaed donkey anti-mouse IgG | Thermo Fisher Scientific | Ref# A21202 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PR20HA | UT Southwestern Protein Chemistry Core |
|
| PR20BAH | UT Southwestern Protein Chemistry Core |
|
| Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl] amine | TClchemicals | Cat#T2993 |
| CuSO4 | Sigma-Aldrich | Cat#451657 |
| Tris(2-carboxyethyl) phosphine hydrochloride | TClchemicals | Cat#T1656 |
| Diazo Biotin-Azide | Click Chemistry Tools | Cat#1041 |
| 1,6-Hexanediol | Sigma-Aldrich | Cat#240117 |
| 2,5-Hexanediol | Sigma-Aldrich | Cat#H11904 |
| 1,5-Pentanediol | Sigma-Aldrich | Cat#76892 |
| 1,4-Butanediol | Sigma-Aldrich | Cat#493732 |
| Aβ fibrils | Dr. Robert Tycko | |
| Sup35NM | Dr. Randal Halfmann | |
| FITC-phalloidin | Sigma-Aldrich | Cat#P5282 |
| tubulin | Cytoskeleton Inc. | Cat#T240-A |
| actin | Cytoskeleton Inc. | Cat#AKL95-B |
| Guanosine-5’-[(α,β)-methyleno]triphosphate | Jena Bioscience | Cat#NU-405 |
| Critical Commercial Assays | ||
| Pierce BCA protein assay kit | Thermo Scientific | Cat#23227 |
| Recombinant DNA | ||
| pCW-3xFlag-PR100-HA | This paper | |
| pMD2.g | Addgene | 12259 |
| pSPAX2 | Addgene | 12260 |
| pHis-Parallel-mCherry-FUS N214 | Kato et al., 2012 | |
| pHis-Parallel-FUS N214 | Kato et al., 2012 | |
| pHis-Parallel-GFP-FUS N214 | Kato et al., 2012 | |
| MBP-PTB-hnRNPA2 LC | Xiang et al., 2015 | |
| pHis-Parallel-GFP-hnRNPA2 LC | Kato et al., 2012 | |
| pHis-Parallel-GFP-hnRNPA1 full-length | This paper | |
| pHis-Parallel-GFP-hnRNPA1 LC | Kato et al., 2012 | |
| pHis-Parallel-GFP-hnRNPA1ΔLC | This paper | |
| pHis-Parallel-GFP-hnRNPDL LC | This paper | |
| pHis-Parallel-mCherry-Neurofilamnet Light/Medium/Heavy Head |
This paper | |
| pHis-Parallel-mCherry-Vimentin Head | This paper | |
| pHis-Parallel-GFP-Peripherin Head | This paper | |
| pET28-Vimentin Full length | This paper | |
| pET28-Vimentin Tailless | This paper | |
| pET28-Vimentin Headless | This paper | |
| pHis-GFP-PR20 | Kown et al., 2014 | |
| pHis-GFP | Kato et al., 2012 | |
| Software and Algorithms | ||
| Graphpad Prism | http://www.graphpad.com/scientific-software/prism/ | |
| SEG Prediction of low complexity regions | IMP Bioinformatics Group |
www.biology.wustl.edu/gcg/seg.html |
| VCCLAb | www.vcclab.org/ | |
| ACD/Labs | www.acdlabs.com | |
| EPISuite | www.epa.gov/tsca-screening-tools/epi-suitetm-estimation-program-interface | |
| ChemAxon | www.chemaxon.com | |