Figure 3.

The effects of chronic BHB exposure on astrocytic glycolysis are reversible. Cultures were pre-incubated with 2 mM BHB for 3 days and then sequentially exposed to 12 mM K⁺, 5 mM azide and the GLUT blocker cytochalasin B (20 µM). After transiently withdrawing glucose for calibration purposes, cultures were superfused with a buffer devoid of BHB, followed by reapplication of K⁺, azide and cytochalasin. Bars represent the average extent of glucose depletion measured at the end of the stimulations in three separate experiments (20–30 cells per experiment). Previous experiments showed that glucose metabolism in astrocytes is not affected by transient glucose depletion.²¹