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. 2015 Oct 29;36(10):1813–1822. doi: 10.1177/0271678X15613955

Figure 6.

Figure 6.

Inhibition of astrocytic glycolysis in ketotic mice. (a) Protocol for the estimation of astrocytic glucose consumption in ketotic mice. (b) Effect of food deprivation on body weight and ketone body concentration in urine. (c) Expression of the glucose sensor FLII12Pglu700µΔ6 in astrocytes in acute hippocampal slices. Bar = 100 µm. (d) Measurement of astrocytic glucose consumption rate with 20 µM cytochalasin B in acute hippocampal slices of control (gray symbols) and 36-h fasting mice (open symbols). Bars represent the average rates obtained in three separate experiments (5 –8 cells per experiment).