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. 2016 Oct 19;10:3415–3423. doi: 10.2147/DDDT.S115801

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© 2016 Li et al. This work is published and licensed by Dove Medical Press Limited

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Chemotaxis of the GFP-labeled CR2-sFlt 1 toward complement cleavage fragments in the transwell assay (A–D) and mouse CNV model (E and F).

Notes: (A) GFP signal of CR2-sFlt 1 suspension in the upper chamber before incubation. (B) No GFP signal in the upper chamber suspension after 1 hour incubation. (C) Most increased green signals in the interface membrane of the transwell chamber after 1 hour incubation. (D) Green signals were detected in the lower chamber suspension after 1 hour incubation. (E) Green fluorescence signals in frozen sections of eye tissue in the CR2-sFlt 1-treated group. (F) Green fluorescence signals merged with corresponding HE staining.

Abbreviations: GFP, green fluorescent protein; CNV, choroidal neovascularization; HE, hematoxylin and eosin.