TABLE 1.

Proteins identified in screens for binding partners of GLDPH tandem

Two-hybrid experiments were performed by Myriad Genetics. In proteomic studies, HeLa cell lysates were mixed with LUVs to which the GLDPH fragment of AGAP1 had been adsorbed. The mixture was mixed with sucrose and layered in a discontinuous sucrose gradient and was subject to centrifugation. Proteins that associated with LUVs that floated through the sucrose gradient were analyzed by mass spectrometry. MPRIP, myosin phosphatase Rho-interacting protein.

	AGAP as bait in two-hybrid experiments	AGAP as prey in two-hybrid experiments	Proteomics
AGAP1	Rock1, Calcoco2, RhoA, Cdc42, Rac1	PLEKHB1	Dynactin subunit 1, Kif5, myosin 1c, Rab11, actin
AGAP2	Rock1, AGAP1, α-actinin, filamin G, MPRIP	PLEKHB1, Rab5, Rab2
AGAP3	Kif5B, clathrin heavy chain, PI-4kinaseA