FIGURE 5.

TrkA CIPA mutations induce protein misfolding and aggregation. A, trypsin digestion of Triton X-100-soluble fractions of TrkA mutants. Samples were incubated with the indicated concentrations of trypsin during 10 min on ice, quenched by adding SDS-PAGE sample buffer (2 times), boiled, and analyzed by SDS-PAGE/immunoblot with a specific antibody against the extracellular region of TrkA. B, analysis of disease protein solubility in the non-ionic detergent Triton X-100. Cell extracts were prepared in lysis buffer supplemented with 1% Triton X-100. Total, pellet, and supernatant were analyzed by SDS-PAGE and immunoblotting. C, the ratio of pellet to total (pellet/total) was quantified for two independent experiments. Error bars are S.E. WB, Western blot.