TABLE 1.

Allosteric deoxynucleotide activators of SAMHD1 have only a minor effect on substrate specificity

Catalytic rates were measured for different combination of allosteric and substrate deoxynucleotides. Only dCTP hydrolysis displays sensitivity to the allosteric ligand (indicated by bold values), but the effect is only about 2-fold.

dNTP in A2 site	Substrate hydrolysis rates for WT				Substrate hydrolysis rates for T592D
	dGTP	dCTP	dATP	dTTP	dGTP	dCTP	dATP	dTTP
	s−1				s−1
dGTP	2.23	1.77	3.34	3.02	2.57	0.77	2.53	2.45
dCTP	1.99	1.89	2.78	3.15	2.40	0.66	3.03	3.08
dATP	1.96	2.31	2.93	2.74	2.56	1.78	2.33	2.91
dTTP	2.11	1.79	3.07	3.19	2.33	0.39	1.29	1.73