FIGURE 7.

Effects of glucose or insulin stimulation on FoxO1 localization in β-cells. After starvation, control or βIRKO β-cell lines were treated with vehicle (PBS), PI3K inhibitor LY294002, high glucose (450 mg/dL), or insulin (10 nm) for 30 min. A, representative pictures of β-cells immunostained for FoxO1 (red) and DAPI (blue). The scale bar indicates 20 μm. B, the proportions of nuclear FoxO1-positive cells in control (filled bars) or βIRKO (empty bars) β-cell line. Data are mean ± S.E. *, p < 0.05 compared with respective controls.