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. 2016 Aug 12;291(41):21496–21509. doi: 10.1074/jbc.M116.734756

FIGURE 2.

FIGURE 2.

Expansion of GICs induced by Tsc1 deletion is independent of growth factors. A, Western blotting to detect the indicated proteins in control and Tsc1Δ/Δ cells. Deletion of the Tsc1 gene in vitro was induced with 4-OHT treatment to prepare Tsc1Δ/Δ cells (see “Experimental Procedures”). Lysates were prepared from control and Tsc1Δ/Δ cells treated with/without EGF + FGF2 for 24 h. Actin was a loading control. B, quantitation of sphere formation of control and Tsc1Δ/Δ cells cultured with/without EGF + FGF2. Data are the mean sphere number (left) and size (right) ± S.D. (error bars). C, Western blotting to detect Olig2 in control and Tsc1Δ/Δ cells cultured without EGF + FGF2 for 5 days. D, quantitation of sphere formation by control and Tsc1-deficient huKO+ cells cultured with EGF + FGF2 and treated with/without the indicated concentrations of the EGFR inhibitor gefitinib. Data are the mean ± S.D. **, p < 0.01; ***, p < 0.001.