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. 2016 Aug 12;291(41):21496–21509. doi: 10.1074/jbc.M116.734756

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effects of Tsc1 deficiency on mitochondrial function and sphere formation in mouse glioma cells. A, quantitation of OCR by control and Tsc1-deficient cells cultured without EGF + FGF2. Data are the mean ± S.D. (error bars). B, qRT-PCR analysis of mitochondrial DNA content in the cells in A. Data were normalized to genomic DNA and are presented as the mean -fold change ± S.D. relative to control cells. C, qRT-PCR analysis of Atp5g1, Cox5a1, and cytochrome c mRNA levels in the cells in A. Data are the mean -fold change ± S.D. relative to control cells. D and E, quantitation of intracellular ATP levels in control and Tsc1-deficient cells that were cultured without EGF + FGF2 and treated with the ATP synthetase inhibitor oligomycin (D) or glucose withdrawal (E) for 12 h. Data are the mean -fold change ± S.D. relative to control cells. F, quantitation of sphere formation by control and Tsc1-deficient cells that were cultured without EGF + FGF2 and treated with the indicated concentrations of oligomycin. Data are the mean ± S.D. **, p < 0.01; ***, p < 0.001.