FIGURE 7.

Effects of selected small molecule compounds on mitochondrial functions. A, quantitation of intracellular ATP levels in human GBM patient-derived TGS-01 cells that were treated with the indicated concentrations of the indicated compounds for 6 h. Data are the mean -fold change ± S.D. (error bars) relative to untreated control cells. B, analysis of mitochondrial membrane potential in TGS-01 cells that were treated for 30 min with the indicated concentrations of the indicated compounds, followed by determination of mitochondrial membrane potential using JC-10. Representative flow cytometric data for cells with (red line) or without (blue line) drug treatment are shown. C, quantitation of changes in OCR in AGS cells that were treated first with oligomycin to inhibit ATP synthetase activity and then with DMSO (vehicle control), nigericin (1 μm), or valinomycin (1 μm) (n = 3). Data are mean ± S.D. percent of OCR (n = 3). **, p < 0.01; ****, p < 0.0001; ns, not significant.