TABLE 3.

Conformation of α-1,4 linkages in saccharides bound to S. coelicolor GlgE isoform 1

Glucose residue	Subsite	2-3′a	O5-C1-O1-C4′b ϕ	C1-O1-C4′-C5′b ψ
		Å	°	°
Crystalline α-maltosec	NAd	2.8	116	−118
Malto-octaose (product)
1, non-reducing end	−2	4.0	77	−151
2	−1	4.9	34	−157
3	+1	4.7	48	−156
4	+2	4.2	59	−152
5	+3	4.1	68	−140
6	+4	3.2	100	−117
7	+5	3.1	100	−121
8, reducing end	+6	NA	NA	NA

^a The distances between the oxygen atoms at the 2- and 3′-positions of adjacent non-reducing and reducing end residues, respectively, are shown.

^b The torsion angles (as defined by Damager et al. (26)) relate to the structure shown in Figs. 4 and 5.

^c The values associated with crystalline α-maltose are shown for comparison (44).

^d NA, not applicable.