SCHEME 1.
Proposed catalytic scheme for RIFMO. The left-hand side of the scheme depicts the steps involved in the binding of RIF and the reduction of the FAD by NADPH (reductive half-reaction). The right-hand side shows the steps involved in the hydroxylation of RIF and regeneration of the oxidized FAD (oxidative half-reaction). Downward arrows denote substrate binding, and upward arrows denote product dissociation. For each step, the redox state of the FAD is listed above the horizontal line. Enzyme-bound substrates and products are indicated below the horizontal line. E-Flox, RIFMO with oxidized FAD; E-Flre, RIFMO with reduced FAD; E-FlOO(H), RIFMO C4a-hydroperoxyflavin intermediates; E-FlOH, RIFMO C4a-hydroxyflavin. Adapted from Ref. 16.