Skip to main content
. 2016 Aug 16;291(41):21584–21595. doi: 10.1074/jbc.M116.730978

FIGURE 2.

FIGURE 2.

KRas is the major Ras isoform downstream of cAMP. A, cells transfected with NS shRNA, shRNA against human KRas, shRNA against human HRas, or shRNAs against both KRas and HRas (KRas/HRas) were treated with F/I for the times indicated. The levels of ERK activation (pERK) and total ERK2 are shown in the 1st two panels. The levels of KRas, total Ras, and HRas are shown in the 3rd, 4th, and 5th panels, respectively. B, cells were transfected with GFP-ERK2 and NS shRNA or shRNA against HRas and either vector or FLAG-tagged shRNA-resistant HRas (FLAG-HRas*). Cells were treated with EGF for 5 min or left untreated, lysed, and subjected to GFP IP. The levels of activation of GFP-ERK2 (pGFP-ERK2) and the levels of total GFP-ERK2 are shown in the 1st and 2nd panels, respectively. The levels of GFP-ERK2 and HRas within the TCL are shown in the bottom two panels.