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. 2016 Aug 16;291(41):21584–21595. doi: 10.1074/jbc.M116.730978

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FIGURE 5. — Ras binds to PDZ-GEF1. A, Hek293 cells were transfected with mCherry-HRasV12 (mCh-RasV12) and either vector (−), FLAG-PDZ-GEF1 (WT), a FLAG-tagged N-terminal fragment of PDZ-GEF1 spanning amino acids 1–716 (N), or a FLAG-tagged C-terminal fragment spanning amino acids 693–1499 (C), and cells were subjected to FLAG IP. The levels of mCh-RasV12 within the IP are shown in the 1st panel. The levels of FLAG-PDZ-GEF1 and fragments within the IP are shown in the 2nd panel. The levels of FLAG-PDZ-GEF1 and fragments within the TCL are shown in the 3rd panel. The levels of mCh-RasV12 within the TCL are shown in the 4th panel. B, cells were transfected with mCherry-HRasN17 (mCh-RasN17) and either vector (−), FLAG-PDZ-GEF1 (WT), the FLAG-tagged N-terminal fragment of PDZ-GEF1 (N), or the FLAG-tagged C-terminal fragment (C), and subjected to FLAG IP. The levels of mCh-RasN17 within the IP are shown in the 1st panel. The levels of FLAG-PDZ-GEF1 and fragments within the IP are shown in the 2nd panel. The levels of FLAG-PDZ-GEF1 and fragments within the TCL are shown in the 3rd panel. The levels of mCh-RasN17 within the TCL are shown in the 4th panel.