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Ad.mda-7 induces the down-regulation of Bcl-x(L) in NSCLC cells, but not in non-transformed lung epithelial cells. The indicated cells (1.2 × 105) were transduced with 150 MOI of either Ad.mda-7 or Ad.CMV control virus. After 48 h, total protein was extracted and subjected to SDS-PAGE analysis/Western immunoblotting for Bcl-x(L) and β-actin. A, A549 cells. B, H838 cells. C, HBEC-3KT cells. Data are representative of four separate determinations on two separate occasions.
