Specific down-regulation of Bcl-x(s) mRNA significantly inhibits the loss of Bcl-x(L) protein induced by Ad.mda-7 and Ad.Bcl-x(s).
A, A549 cells (1.2 × 105) were transfected with Bcl-x(s) (siBcl-x(s), 100 nm) or control siRNA (siCON, 100 nm) and transduced with 150 MOI of either Ad.mda-7 or Ad.CMV control virus. After 48 h, total protein was extracted. Total protein was subjected to SDS-PAGE analysis and Western immunoblotting for Bcl-x(L) and β-actin. Data are representative of six separate determinations on two separate occasions. B, A549 cells (1.2 × 105) were transfected with Bcl-x(s) (100 nm) or control siRNA (100 nm) and transduced with 500 MOI of either Ad.Bcl-x(s) or Ad.CMV control virus. After 24 h, total protein was extracted. Total protein was subjected to SDS-PAGE analysis and Western immunoblotting for Bcl-x(L)/(s) and β-actin. * = nonspecific band or a different Bcl-x splice variant (Bcl-xγ). Data are representative of six separate determinations on two separate occasions.