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. 2016 Aug 29;291(41):21771–21785. doi: 10.1074/jbc.M116.726364

FIGURE 2.

FIGURE 2.

GC-induced MDSC accumulation is dependent on ATF3 deficiency. A and B, WT and ATF3−/− mice were injected with dexamethasone (Dex) or DMSO (Veh) for 5 days (n = 6). A, levels of MDSC subsets in BM, spleen (SP), peripheral blood (PB), and liver were evaluated by flow cytometric analysis. B, population of DC (CD11c+MHCII+) and macrophage (CD11b+F4/80+) subsets in BM were evaluated by flow cytometric analysis. A and B, left flow panels are representative results, and the right graphs were mean ± S.E. from all mice analyzed. C, BM cells were cultured in medium containing GM-CSF, in the presence of Dex or Veh. The frequencies of MDSCs (CD11b+Gr1+) and DCs (CD11c+MHCII+) were analyzed by flow cytometry on day 5. Both representative results (left) and mean ± S.E. from three independent experiments (right) are shown. *, p < 0.05, unpaired t tests.