FIGURE 3.

Expression of hENDOV mRNA. Human ENDOV transcript levels were measured by qRT-PCR in HeLa, T-REx 293, and HAP1 cells treated with or without arsenite (Ars) or nitrite (N). Values relative to GAPDH were calculated and the results presented are the average of 6 qRT-PCRs using cDNA from three independent experiments. Error bars represent standard deviations and statistical significance was determined by Student's t test; ***, p < 0.002. Total RNA used as starting material for cDNA synthesis was analyzed by denaturing agarose gel electrophoresis (samples loaded in the same order as shown for the qRT-PCR). Marker (M) is the Millenium RNA marker (Ambion).