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. 2016 Aug 29;291(41):21786–21801. doi: 10.1074/jbc.M116.730911

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Stress granule assembly is independent of hEndoV activity. A, T-REx 293 cells ectopically expressing FLAG-hEndoV(D52A) were cultured in the absence or presence of arsenite (0.5 mm, 30 min) before processing for visualization of hEndoV (green) and G3BP (red). Nuclei were counterstained using DAPI (blue) and colocalization (yellow) is shown in Merge. Localization of proteins was observed by confocal microscopy (Leica SP8) using a ×40 oil objective. Bar, 5 μm. B, Manders' coefficients for colocalization of FLAG-hEndoV(D52A) and G3BP and C, the average number and size of stress granules per cell in A. Error bars represent the S.D. calculated from 20 randomly selected cells in each cell type. Stress granules were defined as G3BP foci measuring >0.1 μm. Representative images are shown. D, stress granules were induced in HAP1wt and ENDOV⁻ cells and analyzed as above (E).