FIGURE 8.

ADAR1 and Tudor-SN colocalize with hEndoV in stress granules. T-REx 293 cells ectopically expressing FLAG-hEndoV were cultured in the presence or absence of arsenite (0.5 mm, 30 min). Subsequently, cells were fixed and immunofluoroscence experiments were performed using hEndoV (green) and (A) ADAR1 (red) or (B) Tudor-SN (red) antibodies. DRAQ5 (blue) was used to counterstain nuclei, and colocalization (yellow) is shown in Merge. Localization of proteins was observed by confocal microscopy (Zeiss) using a ×63 oil objective.