Impact of recombinant troponin on force-[Ca2+] relationships. Normalized force-[Ca2+] relationships were recorded on human recombinant troponin-exchanged muscles containing WT (black circles; n = 12), RCM (green squares; n = 7), PKC (red up triangles; n = 9), or PKA (blue down triangles; n = 8) (A). B, WT (transcribed from A), RCM + PKA (purple up triangles; n = 6), RCM + PKC (orange squares; n = 5), and PKA + PKC (brown down triangles; n = 6). Experiments were performed at 25 °C. Data are presented as mean ± S.E. Error bars reflect S.E.