Table 3. Properties of influenza viruses isolated from autopsy material in 2015–2016 epidemic season.
| Region of sampling* | Virus | Date of sample collection | Sex | Age | Date of vaccination | Passage (MDCK) | HI titer with ferret anti- A/California/07/2009 | HI titer with human serum after vaccination with “Grippol” | The drug concentration required to reduce NA activity by 50%, IC50, nМ | Sequences published in GISAID | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Oseltamivir | Zanamivir | HA | NA | NS | |||||||||
| Reference antigen | |||||||||||||
| A/California/07/2009 (CDC, Atlanta, the United States) | 2560 | 640 | 0.29 | 0.47 | |||||||||
| Influenza antigen A/California/7/ 2009 (NIBSC, the United Kingdom) | 2560 | - | - | - | |||||||||
| XW (Oseltamivir resistant) [8] | 84.2–280.6 | 0.2–0.8 | |||||||||||
| OSAKA (Zanamivir resistant) [8] | 0.1–0.5 | 123.7–334.5 | |||||||||||
| А(H1N1)pdm09isolated virus | |||||||||||||
| 1 | A/Alania/03/2016 | 06.01.2016 | f | 4 | no | 1 | 2560 | 160 | 0.50 | 0.63 | EPI694640 | EPI694641 | EPI694642 |
| 1 | A/Stavropol/07/2016 | 07.01.2016 | f | 53 | no | 1 | 2560 | 160 | 0.21 | 0.75 | EPI704203 | EPI704204 | EPI704205 |
| 1 | A/Stavropol/11/2016 | 07.01.2016 | f | 27 | no | 1 | 2560 | 160 | 0.52 | 0.72 | EPI704030 | EPI704031 | EPI704032 |
| 1 | A/Kaluga/13/2016 | 01.02.2016 | m | 66 | 30.10.2015 | 1 | 640 | 80 | 0.20 | 0.37 | EPI772567 | EPI772568 | EPI772569 |
| 1 | A/Saint-Petersburg/10/2016 | 03.01.2016 | m | 36 | no | 1 | 2560 | 160 | 0.51 | 0.70 | EPI700033 | EPI700034 | EPI700035 |
| 1 | A/Saint-Petersburg/22/2016 | 08.01.2016 | m | 41 | no | 1 | 2560 | 160 | 0.75 | 0.90 | EPI711130 | EPI748416 | EPI711131 |
| 1 | A/Saint-Petersburg/31/2016 | 01.01.2016 | m | 46 | no | 1 | 2560 | 320 | 0.37 | 0.55 | EPI711128 | EPI749107 | EPI711129 |
| 1 | A/Saint-Petersburg/39/2016 | 17.01.2016 | m | 41 | no | 1 | 2560 | 320 | 0.72 | 1.10 | EPI711208 | EPI749108 | EPI711209 |
| 1 | A/Saint-Petersburg/41/2016 | 18.01.2016 | m | 61 | no | 1 | 1280 | 320 | 0.88 | 1.03 | EPI711210 | EPI749109 | EPI711211 |
| 1 | A/Saint-Petersburg/46/2016 | 17.01.2016 | m | 76 | no | 1 | 2560 | 160 | 0.56 | 0.76 | EPI721829 | EPI721830 | EPI721831 |
| 1 | A/Saint-Petersburg/50/2016 | 25.01.2016 | m | 45 | no | 1 | 1280 | 160 | 1.06 | 1.23 | EPI748389 | EPI748390 | EPI748391 |
| 2 | A/Orenburg/05/2016 | 09.01.2016 | m | 57 | no | 1 | 2560 | 320 | 0.41 | 0.50 | EPI704206 | EPI704207 | EPI704208 |
| 2 | A/Noyabrsk/3/2016 | 11.02.2016 | f | 55 | no | 1 | 1280 | 160 | 0.24 | 0.47 | EPI772605 | EPI772606 | EPI772608 |
| 2 | A/Novosibirsk/181/2016 | 13.02.2016 | m | 59 | no | 1 | 1280 | 160 | 0.62 | 0.75 | EPI772612 | EPI772613 | EPI772614 |
| 3 | A/Chita/317/2016 | 28.01.2016 | f | 48 | no | 1 | 1280 | 160 | 0.68 | 0.80 | EPI772586 | EPI772587 | EPI772588 |
| А(H3N2)isolated virus | |||||||||||||
| Region | A(H3N2) virus | Sampling | Sex | Age | Vaccination | Passage | HI titer with A/Switzerland/9715293/2013 | HI titer with A/HongKong/4801/2014 | Oseltamivir | Zanamivir | HA | NA | NS |
| Reference antigen | |||||||||||||
| A/Switzerland/9715293/13(H3N2) | 1280 | 80 | 0.62 | 0.75 | |||||||||
| A/HongKong/4801/2014 (H3N2) | 80 | 640 | 0.78 | 0.93 | |||||||||
| 1 | A/Saratov/5/2016 | 11.01.2016 | m | 61 | no | 2 | 80 | 640 | 0.67 | 0.40 | EPI687295 | EPI687829 | EPI693968 |
* Regions of sampling: 1 –European Russia, 2 –Urals and Western Siberia, 3 –Eastern Siberia and Russian Far East.