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. 2016 Oct 24;11(10):e0161445. doi: 10.1371/journal.pone.0161445

Fig 2. Tyrosine disrupts internalization into early endosomes.

Fig 2

SN56 cells were transfected with βAPP-CFP (with or without tyrosine mutations) and Rab5-mRFP. The HA-tag on our βAPP-CFP construct was fluorescently labeled using an anti HA-Zenon conjugate. a-d) Representative images of βAPP-CFP, bearing one of the tyrosine mutations, internalized into Rab5-mRFP compartments after 15-minutes. The edge of the cell is shown by the white border, and was drawn based on white-light images. Triangles point to colocalized pixels. Scale bars represent 5μm. e) The percentage of APP co-localized with Rab5 was quantified using Imaris and graphed (* = p<0.05, error bars represent SEM).