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. 2016 Oct 24;11(10):e0161445. doi: 10.1371/journal.pone.0161445

Fig 4. Tyrosine motif mutations affect on APP/AP-3 interaction.

Fig 4

SN56 cells were transfected with plasmids expressing wild type APP or APP with mutations Y709A, Y738A, or Y743A. Cells were fixed and iPLA was performed to detect interaction between APP with AP-3δ. a) Representative images are shown. The white border shows edge of the cell and was drawn based on the white light images. Scale bars represent 5μm. b) The dots per cell was counted using Imaris, normalized to cell volume, and graphed in Prism 5.0b (p<0.05). SN56 cells were transfected with plasmids expressing wild type APP or APP bearing phosphomimetic (S711E) or dephosphomimetic (S711A) mutations. Cells were fixed and iPLA was performed to determine if there was an interaction between APP and AP-3. c) Representative images of APP/AP-3δ interaction. The white border shows edge of the cell and was drawn based on the white light images. Scale bars represent 5μm. d) The number of spots per cell was counted and normalized to cell volume. Error bars represent SEM and * = p<0.05.