. 2016 Oct 24;11(10):e0164973. doi: 10.1371/journal.pone.0164973

Table 1. Bacterial strains and plasmids used in this study.

Strains or plasmids	Characteristics	Reference or source
Bacterial strains
E. coli EC1000	RepA⁺ MC1000, kanamycin resistance, carrying a single copy of the pWV01 repA gene; host for pORI28-based plasmids	[14]
E. coli ECHEA	E. coli EC1000 containing plasmid pORIHEA	This study
E. coli MC1061	Plasmid-free strain; araD 139, Δ(ara-leu)7697, Δ(lac)X74, galU^-, galK^-, hsdR^-, rpsL	[15]
L. lactis NZ9000	Plasmid-free strain; MG1363 pepN: nisRK	[1]
L. plantarum LB-B1	Pediocin PA-1 producing strain (isolated from fermented dairy product)	[13]
L. plantarum WQ0815	Pediocin PA-1-sensitive strain (isolated from Sichuan Pickle)	Laboratory collection
L. mesenteroides 05–43	Pediocin PA-1-sensitive strain(isolated from fermented dairy product)	Laboratory collection
L. acidophilus 05–172	Pediocin PA-1-sensitive strain (isolated from fermented dairy product)	Laboratory collection
L. salivarius REN	Pediocin PA-1-sensitive strain (isolated from the feces of a healthy centenarian)	[16]
L. plantarum W903	man-PTS EII mutant of L. plantarum WQ0815; Em^r	This study
Plasmids
pTRK669	Ori (pWV01), Cm^r, RepA⁺	[17]
pORI28	Em^r, ori (pWV01), replicates only with RepA provided in trans	[14]
pORIHEA	2.3 Kb; pORI28 with 725-bp internal man-PTS IIAB fragment of L. plantarum	This study
pNZ8148	Gene expression vector, P_nisA, Cm^r	[1]
pNZ8300	5.4 Kb; pNZ8148 with nisin regulatory genes nisRK	This study