Table 2. PCR primers for amplifying genes IIC and IID.
| Primer | DNA sequencea |
|---|---|
| lpcF | 5’-CATGCCATGGGCATGAATTTGAACGTAATTC-3’; NcoI |
| lpdR | 5’-CCGCTCGAGCCCACGAGTCCATCCTTT-3’; XhoI |
| lmcF | 5’-CATGCCATGGCTATGTCTGTTATTGCGATAG-3’; NcoI |
| lmdR | 5’-CCGCTCGAGGCCAGATTGACAAGCACT-3’; XhoI |
| lscF | 5’-ACATGCATGCATGAGTACGATTCAAATT-3’; SphI |
| lsdR | 5’-CCGCTCGAGTTATAGTAAACCAATTAC-3’; XhoI |
| lacF | 5’-CATGCCATGGGAATGAACGCTATACAAATG-3’; NcoI |
| ladR | 5’-CCGCTCGAGTTAAAGAATGTGCCATACG-3’; XhoI |
| lpmcR | 5’-CCATCATCTTGCCCCTTTCTGCAGCTAATACTTGTCGATAAT-3’ |
| lpmdF | 5’-ttatcgacaagtatTAGCTGCAGAAAGGGGCAAGATGATGG-3’ |
| lmpcR | 5’-CTCAGTTTGCCTCCTTCTCTGCAGCTAGTACTTGTTCAAAAT-3’ |
| lmpdF | 5’-ATTTTGAACAAGTACTAGCTGCAGAGAAGGAGGCAAACTGAG-3’ |
| lpscR | 5’-gtttttcccctcctaagCTAATACTTGTCGATAAT-3’ |
| lpsdF | 5’-ttatcgacaagtatTAGcttaggaggggaaaaac-3’ |
| lspcR | 5’-ctcagtttgcctccttctTTAGATGTCTTCCAAAAT-3’ |
| lspdF | 5’-attttggaagacatcTAAagaaggaggcaaactgag-3’ |
| lpacR | 5’-ctcctctacgcgttcattCTAATACTTGTCGATAAT-3’ |
| lpadF | 5’-ttatcgacaagtatTAGaatgaacgcgtagaggag-3’ |
| lapcR | 5’-CTCAGTTTGCCTCCTTCTttaataatcatcaatga-3’ |
| lapdF | 5’-tcattgatgattatTAAagaaggaggcaaactgag-3’ |
aRestriction site sequences are underlined.