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. 2016 Oct 24;12(10):e1006361. doi: 10.1371/journal.pgen.1006361

Fig 5. New gst-4 regulators identified through genome wide RNAi screening function upstream of wdr-23 and skn-1.

Fig 5

Animals integrated with Pgst-4::GFP were fed bacteria producing dsRNA to positive hits obtained from the RNAi screen and exposed to juglone. (A) Pgst-4::GFP fluorescence was scored and representative images are shown. For Pgst-4::GFP scoring, low refers to little to no GFP signals observed throughout the worm, medium refers to GFP signals observed at the anterior and posterior ends of the worm, and high refers to GFP signals observed throughout the body. n = 50–118 worms. (B) Pgst-4::GFP reporter scoring in either a wdr-23 loss of function (tm1817) or a skn-1 gain of function allele (k1023) mutant background. n = 54–75 worms. *P<0.05, **P<0.01 and ***P<0.001 relative to corresponding control as determined by Chi-Square tests; note that pad-1(RNAi) in skn-1(k1023) could not be tested for significance because it had zero worms with low or medium fluorescence.