Skip to main content
. 2016 Oct 24;12(10):e1005916. doi: 10.1371/journal.ppat.1005916

Fig 6. Knockdown of Fascin does not affect cell aggregation but decreases cell adhesion of MT-2 cells in co-cultures with Jurkat T-cells.

Fig 6

(A) Scheme of immunofluorescence stains with MT-2 cells and Jurkat T-cells. (A-D) MT-2 cells with stable repression of Fascin (shFascin5) or control cells (shNonsense) were co-cultured with Calcein-stained Jurkat T-cells for 1h at 37°C either on poly-L-lysine- (a-e, k-o) or on fibronectin-coated (f-j, p-t) coverslips. (B) Immunofluorescence stainings of co-cultures. Jurkat cells were pre-stained with Calcein-AM (green) to differentiate between the two cell types. Stainings of gag (blue), Fascin (red) and the merge of all three stainings are shown. Transmitted light served as control. Representative stainings of three independent experiments are shown. (C-D) Results of automatic image analysis (see S1 Fig). The means of three independent experiments ± standard error (SE) are shown and were compared to shNonsense using Student’s t-tests (*: p<0.05, **: p<0.01). (C) Percentage of MT-2 cells (shNonsense, shFascin5) with 0 to ≥5 cell-to-cell contacts to co-cultured Jurkat T-cells on the different coatings. (D) Percentage of adherent MT-2 cells (shNonsense, shFascin5) on the different coatings normalized on MT-2 shNonsense.