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. 2016 Oct 24;11(10):e0165158. doi: 10.1371/journal.pone.0165158

Fig 1. Effect of GlcN on IL-8 production and O-GlcNAc modification in MH7A cells.

Fig 1

MH7A cells were incubated without (-) or with (+) 15 pg/ml IL-1β for 13h in the absence or presence of GlcN (1, 2 or 5 mM). IL-8 in the culture supernatants was measured by ELISA (A). Data are mean ± S.E. of four to five separate experiments. Values were compared between the absence and presence of GlcN. MH7A cells were incubated without (-) or with (+) 15 pg/ml IL-1β for 13h in the absence or presence of GlcN (5 mM) or GlcN+alloxan. The culture supernatants and cells were recovered, and O-GlcNAc-modified proteins and GAPDH (GAPDH) was evaluated by western blotting (B), whereas IL-8 in the culture supernatants was measured by ELISA (C). The image is representative of five separate experiments. Arrows a and b indicated 17 and 8 kDa positions, respectively (B). Data are mean ± S.E. of five separate experiments (C). Values were compared among IL-1β-stimulated cells without or with GlcN or GlcN+alloxan. *p<0.05, **p<0.01, ***p<0.005.