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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Nat Microbiol. 2016 Aug 8;1(10):16134. doi: 10.1038/nmicrobiol.2016.134

Fig. 1. Stability of FlgE high molecular weight complexes (HMWCs) derived from PFs periplasmic flagella (PFs), polyhooks (PHs), and rFlgE.

Fig. 1

a. Representative cryoelectron- microscopy(cryo-EM) images of purified T. denticola PFs and PHs. b. Representative western blot of PFs and PHs using anti-FlgE antibodies, indicating that the HMWCs from PFs were stable to boiling and formic acid (FA). c. Time course of in vitro formation of the HMWC using western blot and (d) Imperial staining. The monomer has a mass of 50 kDa. e. The densitometry plot of the Imperial stained gel indicates that the HMWC (squares) is synthesized with a concomitant decrease of the rFlgE monomer (circles). f. Representative gel indicating the stability of the in vitro synthesized HMWC to boiling and FA.