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. 2016 Oct 24;11(10):e0164773. doi: 10.1371/journal.pone.0164773

Fig 3. Hmt1 affected Scd6 subcellular localization.

Fig 3

(A) Localization of Scd6-mCherry and Edc3-GFP; Wild-type (WT) and hmt1 mutant cells carrying endogenous mCherry-tagged Scd6 were transformed with the pRS314-EDC3-GFP plasmid. Cells were then grown to mid-log phase and were resuspended in medium lacking glucose; Scale bar, 5 μm. (B) Percentages of more than 200 cells with Scd6-mCherry or Edc3-GFP foci from three independent experiments are presented as means ± standard deviations (SD); *, P < 0.05. (C) Scd6-mRFP localization; scd6 cells containing pRS316-SCD6-mRFP, pRS316-SCD6RF-mRFP, or pRS316-SCD6RK-mRFP were grown and observed as in A. (D) Percentages of more than 200 cells with Scd6-mRFP foci from three independent experiments are shown as means ± SD; *P < 0.05.