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. 2016 Oct 24;11(10):e0164773. doi: 10.1371/journal.pone.0164773

Fig 4. Scd6 plays roles in the accumulation of P-bodies.

Fig 4

(A) Foci formation of Dcp2-GFP under glucose depletion condition. Wild-type (WT) and scd6 mutant cells carrying endogenous Dcp2 tagged with GFP were grown to mid-log phase and were resuspended in medium lacking glucose (-Glucose); Scale bar, 5 μm. Percentages of cells with Dcp2-GFP foci among more than 200 cells from three independent experiments are shown as means ± SD. Fluorescence intensities of Dcp2-GFP were measured using the linescan function in MetaMorph, and fluorescence profiles are shown in the right panel. (B) Foci formation of Dcp2-GFP in Scd6–overexpression condition. Dcp2-GFP cells harboring YEplac195 (2μ) or YEplac195-SCD6 (2μ-SCD6) plasmids were grown to mid-log phase in glucose-containing medium (+Glucose) as shown in A; *P < 0.05.