FIGURE 2.
In vivo termination assays with the Rho* mutants. A, sequence of the PRM-trac-racR region of the rac prophage fused upstream of a lacZ cassette. Different regions along with primers used for making the DNA templates are indicated. The transcription start site G, is shown as a small arrow. Promoter and racR regions are highlighted. B, bar diagrams showing the β-galactosidase activities indicating the termination efficiencies of WT and Rho* mutant proteins at the NusG-dependent terminator, trac (6), in the presence of different NusG derivatives. The strain RS1430 (see Table 1) was used in the assays. Rho and NusG mutants together with the reporter construct are indicated. Error bars are obtained from the measurements of 5 independent colonies.