FIGURE 4.

Distributions of the binding rate constant (k_on) for Intact (left), CD (center left), CBM-linker (center right), and CBM (right). The distributions of Intact, CD, CBM-linker, and CBM were fitted with two or three Gaussians. The concentrations of the samples were 25, 250, 50, and 200 pm for Intact, CD, CBM-linker, and CBM, respectively. The number of cellulose microfibrils analyzed was 170, 106, 104, and 193 for Intact, CD, CBM-linker, and CBM, respectively. The bin widths were 1.25 × 10⁸ m⁻¹ μm⁻¹ s⁻¹ for Intact and CBM-linker, 1.4 × 10⁷ m⁻¹ μm⁻¹ s⁻¹ for CD, and 2.0 × 10⁷ m⁻¹ μm⁻¹ s⁻¹ for CBM.