TABLE 3.

Values of K_d calculated from k_on and k_off

Sample	kona	koff	Kdb
	m−1 μm−1 s−1	s−1	m μm
Intact
Low affinity site	5.3 × 108	1.1	0.21 × 10−8
High affinity site	2.3 × 108	0.10	0.044 × 10−8
CD
Low affinity site	3.7 × 107	1.5	4.0 × 10−8
High affinity site	1.5 × 107	0.16	1.1 × 10−8
CBM-linker
Low affinity site	2.5 × 108	2.6	1.0 × 10−8
High affinity site	1.4 × 108	0.14	0.10 × 10−8
CBM
Low affinity site	3.9 × 107	2.3	5.9 × 10−8
High affinity site	1.7 × 107	0.083	0.49 × 10−8

^a The k_on for short and long bindings was calculated from the ratio of the fast and slow components of the k_off.

^b The unit of μm represents the length of a crystalline cellulose microfibril.