FIGURE 1.

Effect of pioglitazone treatment on metabolic parameters in pre-diabetic NOD mice. 6-Week-old pre-diabetic NOD mice (n = 10 per group) were placed on either normal chow (Control) or chow containing 0.01 wt% pioglitazone (Pio). A, body weights during feeding. B, lean body mass as assessed by dual-energy x-ray absorptiometry after 4 weeks of feeding. C, fat mass as assessed by dual-energy x-ray absorptiometry after 4 weeks of feeding. D, percent body fat as assessed by dual-energy x-ray absorptiometry after 4 weeks of feeding. E, results of intraperitoneal GTT after 4 weeks of feeding. F, AUC analysis of GTT shown in panel E. G, results of intraperitoneal insulin tolerance test after 4 weeks of feeding. H, AUC analysis of corresponding ITT in panel G. I, immunoblot analysis of phospho-AKT (Ser-473) and total AKT in liver and muscle isolated from 4-week-fed mice after acute insulin injection. Immunoblots show representative data from three mice per group (Control and Pio). * indicates that the value is significantly different from control by two-tailed t test.