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. 2016 Sep 9;291(43):22524–22533. doi: 10.1074/jbc.M116.741694

FIGURE 2.

FIGURE 2.

Effect of pioglitazone on the frequency and severity of insulitis in pre-diabetic NOD mice. 6- Week-old pre-diabetic NOD mice were placed on either normal chow (Control) or chow containing 0.01 wt% pioglitazone (Pio). After 4 weeks of feeding, pancreas and pancreatic lymph nodes were harvested from animals (n = 5 per group). A, representative images of islets stained for glucagon, insulin, and nuclei (DAPI) from control- and pioglitazone-treated animals; the dense blue nuclei (region indicated by arrows) represent immune infiltrate (insulitis), B, results of insulitis scoring of control- and pioglitazone-treated animals. C, flow cytometric analysis from pancreatic lymph nodes showing Treg cells (CD4+CD25+Foxp3+) as a percentage of total CD4+ cells. D, flow cytometric analysis from pancreatic lymph nodes showing Th1 cells (CD4+IFN-γ+) as a percentage of total CD4+ cells. E, flow cytometric analysis from pancreatic lymph nodes showing Th17 cells (CD4+IL17A+) as a percentage of total CD4+ cells.