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. 2016 Sep 9;291(43):22524–22533. doi: 10.1074/jbc.M116.741694

FIGURE 6.

FIGURE 6.

Effect of pioglitazone on β cell ER stress in pre-diabetic NOD mice. 6-Week-old pre-diabetic NOD mice were placed on either normal chow (Control) or chow containing 0.01 wt% pioglitazone (Pio). After 4 weeks of feeding, pancreas was harvested (n = 5) or islets were isolated and subjected to RT-PCR (n = 3 per group) or immunoblot analysis (n = 3 per group). A, spliced Xbp1 mRNA (relative to Actb mRNA). B, immunoblot analysis and quantitation for ATF4. C, Gadd34 mRNA (relative to Actb mRNA). D, immunoblot analysis and quantitation for Bip. E, Chop mRNA (relative to Actb mRNA). * indicates that the values are significantly different (p < 0.05) by two-tailed t test.