FIGURE 2.

Crystal structure of EntF bound to the MLP YbdZ. The Ser-AVS inhibitor was used to covalently trap the PCP in an interaction with the adenylation domain in the thioester-forming conformation. Shown is the complete terminal module with condensation domain (gray), adenylation N-terminal subdomain (red), adenylation C-terminal subdomain (salmon), PCP (green-cyan), thioesterase domain (blue), and YbdZ (yellow). Black dotted lines represent unresolved coils, including the adenylation domain-PCP linker, the PCP-thioesterase linker, and a loop connecting the two α-helices of the lid on the thioesterase domain.