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AtRBSK localizes to plastids. Confocal microscopy of isolated chloroplasts shows localization of A, chlorophyll; B, AtRBSK-YFP; C, DAPI; D, PEND-CFP; E, overlay of all channels. Not all leaf cells are transformed in transient assays and hence not chloroplasts fluoresce for CFP and YFP. F-H, expression of AtRBSK-YFP and PEND-CFP in roots of stably transformed Arabidopsis thaliana ecotype Col roots. F, AtRBSK-YFP; G, PEND-CFP; H, overlay. Scale bars = 10 μm.
