FIGURE 7.

Complementation of rbsk-2 with a transgene expressing epitope-tagged RBSK. A, immunoblot analysis of 35S:RBSK-MYC expression in seedlings. 50 μg of total protein from each indicated plant line was loaded per lane. Top panel is anti-MYC immunoblot. Bottom panel is Ponceau S-stained membrane as a loading control. B, representative images of 11-day-old seedlings of the indicated genotypes on either GM containing either 1% sucrose (left) or 1% ribose (right). C and D, quantification of the fraction of green seedlings out of germinated seedlings from B, grown on GM containing either C, 1% sucrose, or D, 1% ribose. Three technical replicates were analyzed for each condition. Analysis included three independent biological replicates of seedlings expressing 35S:RBSK-MYC in the rbsk-2 background and one biological replicate of the parental rbsk-2 for comparison. Counts included >20 seedlings per replica. Scatter plots show the fraction of green seedlings on each of the three technical replicates with mean ± S.D. indicated. The fraction of green seedlings was not different from Col for the 3 35S:RBSK-MYC expressing lines and much greater than observed for the rbsk-2 progenitor line. All asterisks indicate statistical difference from WT. ****, p < 0.0001.