FIGURE 4.

In vivo chromatin immunoprecipitation assay using rat visual cortical nuclear extract shows that NRF-2 interacts with the promoter of Bdnf exon at both sites I and II, but not at sites III and IV. Immunoprecipitation was carried out with anti-NRF-2α antibody (NRF-2 lane), anti-nerve growth factor receptor p75 antibody (negative control, NGFR lane), or no antibody (negative control, blank lane). 0.5% of input chromatin (input DNA lane) served as the control for PCR. The positive control for NRF-2 binding was COX6b, whereas exon 8 of NRF-1 was the negative control.