FIGURE 6.

Overexpression of Abi1 increases HCV propagation. A, Huh7.5 cells were electroporated with 10 μg of JFH1-luc RNA. At 48 h after electroporation, cells were transfected with increasing amounts of FLAG-tagged Abi1. Luciferase activities were determined at 48 h after transfection. Protein expressions in the same cell lysates were verified with the indicated antibodies (lower panels). Experiments were carried out in triplicate. Error bars indicate the mean ± S.D. B, Huh7.5 cells were electroporated with JFH1-luc RNA and cotransfected with the same expression plasmids as described in A. Luciferase activities were determined at the indicated time points. Experiments were carried out in triplicate. Error bars indicate the mean ± S.D. C, Huh7.5 cells were transfected with 2 μg of either control vector (−) or FLAG-tagged Abi1 expression plasmid for 24 h, and then either mock infected or infected with Jc1 for 4 h. At 2 days postinfection, total cells lysates were immunoblotted with the indicated antibodies. Experiments were carried out in triplicate.