FIGURE 2.

sHsps prevent α-syn aggregation but, in doing so, do not form a stable high molecular mass sHsp-target protein complex. A and B, size exclusion chromatograms of soluble α-syn (300 μm), αB-c (300 μm), Hsp27 (300 μm), or post-aggregation samples of soluble α-syn (300 μm) in the absence and presence of 1:1 molar ratio of αB-c or Hsp27. C and D, immunoblot analysis of the eluate fractions collected from the size exclusion column after loading with the sample from A or C containing α-syn and αB-c or Hsp27. Aliquots from every fraction (1 ml) collected between 8 and 20 ml were loaded on to a SDS-PAGE gel, transferred to nitrocellulose membrane, and blotted with an anti-αB-c, anti-Hsp27, or anti-α-syn antibody. Results shown are representative of two independent experiments.