FIGURE 4.

Aggregation-prone α-syn induces the dissociation of oligomeric Hsp27. Sedimentation velocity analysis of 0.5 μm CF488-labeled Hsp27 in the absence or presence of increasing concentrations of α-syn (0–50 μm). A, radial scans are displayed for the highest α-syn concentration overlaid with theoretical fits to the c(s) model generated by SEDFIT. Data are collected at 60-min intervals are presented. B, c(s) distributions for each profile are shown, and C, the weight-average sedimentation coefficient was calculated via integration from 1 to 25 S for each concentration of α-syn. Data were then fitted with a linear regression model.