TABLE 2.
Muropeptide composition of C. jejuni wild-type 81-176, Δape1, ΔpatB, and ΔpatA, Δape1C, ΔpatBC, ΔpatAC, and Δoap showing relative abundance of muropeptides corresponding to peaks in HPLCs (Fig. 2)
| Peaksa | Muropeptide | Sample Set #1 |
Sample Set #2 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 81-176 | Δape1 | ΔpatB | ΔpatA | 81-176 | Δape1C | ΔpatBC | ΔpatAC | Δoap | ||
| 1 | Tri | 5.8 | 12.3 | 7.3 | 9.5 | 8.8 | 9.6 | 8.8 | 6.0 | 6.2 |
| b | Tetra-Gly-4 | 0.4 | 0.4 | 0.4 | 0.3 | 0.4 | ||||
| 2 | Tetra | 16.1 | 16.6 | 15.8 | 15.2 | 16.1 | 16.9 | 16.2 | 15.7 | 15.1 |
| 3 | Penta-Gly-5 | 0.6 | 0.8 | 0.7 | 0.7 | 0.8 | 1.0 | 1.2 | 0.9 | 1.0 |
| 4 | Di | 16.3 | 8.3 | 16.1 | 14.3 | 13.0 | 10.1 | 10.4 | 16.2 | 15.7 |
| 5 | Tri-Ac | 0.3 | 2.8 | 0.3 | 0.3 | 1.0 | 1.2 | 0.7 | 0.5 | 0.4 |
| 6 | Tetra-Ac | 0.6 | 2.6 | 0.0 | 0.0 | 0.6 | 0.7 | 0.5 | 0.0 | 0.0 |
| 7 | Di-Ac | 0.0 | 1.9 | 0.0 | 0.0 | 0.2 | 0.5 | 0.5 | 0.6 | 0.4 |
| 8 | TetraTri | 9.7 | 7.6 | 11.1 | 13.7 | 10.5 | 10.1 | 10.6 | 11.5 | 11.2 |
| 9 | TetraPenta-Gly-5 | 0.4 | 0.7 | 0.6 | 1.0 | 0.8 | 0.9 | 1.0 | 0.8 | 1.2 |
| 10 | TetraTetra | 19.0 | 17.0 | 18.0 | 21.2 | 21.4 | 22.4 | 23.0 | 22.4 | 22.5 |
| 11 | TetraPenta | 0.0 | 0.1 | 0.0 | 0.0 | |||||
| 12 | TetraTri-Ac | 1.8 | 3.4 | 1.6 | 0.0 | 1.5 | 1.7 | 0.9 | 0.0 | 0.0 |
| 13 | TetraTetraTri | 0.8 | 0.5 | 0.9 | 1.5 | 1.0 | 0.9 | 1.3 | 1.3 | 1.4 |
| 14 | TetraTetra-Ac | 0.3 | 1.1 | 0.0 | 0.0 | 1.7 | 1.8 | 1.4 | 0.4 | 0.0 |
| 15 | TetraTetraTetra | 2.9 | 7.0 | 1.9 | 2.8 | 2.5 | 2.8 | 3.1 | 2.9 | 3.4 |
| 16 | TetraTriAnh I | 1.2 | 0.5 | 1.3 | 1.3 | 1.1 | 1.0 | 1.1 | 1.3 | 1.2 |
| 17 | TetraTriAnh II | 2.6 | 1.6 | 2.5 | 2.7 | 2.4 | 2.1 | 2.2 | 2.5 | 2.5 |
| 18 | TetraTetraAnh I | 3.0 | 1.8 | 2.8 | 2.7 | 2.8 | 2.8 | 3.0 | 3.1 | 3.0 |
| 19 | TetraTetraAnh II | 5.6 | 3.7 | 4.9 | 4.9 | 5.2 | 5.0 | 5.1 | 5.4 | 5.5 |
| b | TetraTetraTriAnh | 1.1 | 0.9 | 1.3 | 1.4 | 1.7 | ||||
| 20 | TetraTetraTetraAnh | 4.3 | 1.9 | 3.9 | 4.3 | 4.1 | 4.2 | 4.9 | 4.5 | 5.3 |
| 1–20 | All knownc | 91.2 | 92.3 | 89.5 | 95.9 | 96.8 | 97.1 | 97.4 | 97.7 | 97.9 |
a Peak numbers correspond to those from HPLCs in Fig. 2. Muropeptides are named according to Glauner et al. (88) and are depicted in Fig. 2H. Di, disaccharide dipeptide (disaccharide = β1,4-linked N-acetylglucosamine-N-acetylmuramic acid); Tri, disaccharide tripeptide; Tetra, disaccharide tetrapeptide; Penta, disaccharide pentapeptide Muropeptides 1–7 are monomeric, and muropeptides 8–20 are dimers and trimers connected by peptide cross-links. Modifications: Gly, glycine in position 5 of a peptide side chain; Ac, O-acetyl groups at the C-6 hydroxyl group of MurNAc; Anh, 1,6-anhydro-N-acetylmuramic acid.
b Peak was previously unidentified in Sample Set #1.
c The total abundance does not add up to 100% due to the presence of peaks for which a structure has not been assigned.